Computational 3D Fluorescence Microscopy

We describe a compact and inexpensive computational microscope that encodes 3D information into a single 2D sensor measurement, then exploits sparsity to reconstruct the volume with good resolution across a large volume. Our system uses simple hardware and scalable software for easy reproducibility and adoption. The inverse algorithm is based on large-scale nonlinear optimization with self-calibration of aberrations and we discuss computational optical design approaches for optimizing the syste